Human Adenosine Triphosphate ELISA kit

E01A0038 Human Adenosine Triphosphate ELISA kit

Human Adenosine Triphosphate ATP ELISAkit is suitable for the detection of samples from human species. Adenosine Triphosphate can also be called Adenosine-5'-Triphosphate, ATP.

Specifications of Human Adenosine Triphosphate ATPELISA kit

Product Information
	E01A0038
Product Name	Human Adenosine Triphosphate ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	50-1000 ng/mL
Sensitivity
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Principle of the Assay

ATP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ATP antibody and an ATP-HRP conjugate. The assay sample and buffer are incubated together with ATP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATP concentration since ATP from samples and ATP-HRP conjugate compete for the anti-ATP antibody binding site. Since the number of sites is limited, as more sites are occupied by ATP from the sample, fewer sites are left to bind ATP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATP concentration in each sample is interpolated from this standard curve.

Quality Control on Human Adenosine Triphosphate ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	94-106%
Linearity	Diluent Ratio	Range %
	1:02	93-101
	1:04	90-107
	1:08	92-105
Specificity/Cross-reactivity	No significant cross-reactivity or interference between ATP and analogues was observed.

Citations of Human Adenosine Triphosphate ELISA kit

E01A0038 has been referenced in the below publications:

The research of the metabolism and in vivo functional changes of erythrocyte stored at -65℃.

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