Recombinant Carboxypeptidase B（with His Tag）
CAS: 9025-24-5
Recombinant carboxypeptidase b（with His Tag） is a lyophilized powder that is isolated and purified from recombinant Pichia pastoris and then freeze-dried, and has the same enzyme activity and specificity as natural carboxypeptidase B.
Recombinant Carboxypeptidase B （with His Tag）Specifications
|
Product Name |
Recombinant Lysyl Endopeptidase (Biochemical Grade) |
|
Appearances |
White or off-white powder |
|
Specific Activity |
≥80U/mg pro |
|
MW |
36kD±3.6kD |
|
Purity |
≥95%（SDS-PAGE） |
|
Protein Content |
20%-60% |
|
Residue DNA |
≤100ng/mg pro |
|
Residue HCP |
≤100ng/mg pro |
|
Optimum pH |
|
|
Solubility |
Soluble in buffer |
|
Storage Conditions |
-15°C below, protected from light，sealed. |
The data should be subject to test report of products.
Carboxypeptidase B Application
Production of various proteins or polypeptides, such as insulin and analogs, cosmetic peptides, antimicrobial peptides, food flavor peptides, and the like.
Quality testing of antibodies.
Determination of the C-terminal amino acid of a protein
Carboxypeptidase Aand BRecommended Method
Protein preparation: The protein was dissolved in a solution of 25 mM Tris-HCl, pH 7.6.
Enzyme preparation: The desired enzyme powder was weighed and dissolved in 25 mM Tris-HCl (pH 7.6) to give an enzyme solution concentration of 1~10 mg/ml.
Reaction recommended enzyme amount: target protein (mass ratio) = 1:50 ~ 1:1000, optimum pH is 7.0~9.0.
Carboxypeptidase B Transport and Storage Stability
Transportation stability: It is transported in an ice pack to keep it active.
Storage stability: The lyophilized powder can be stored for at least 24 months at -15°C below.
25 mM Tris-HCl (pH 7.6) was dissolved and stored at -15°C below, and repeatedly frozen and thawed 10 times without loss of activity.
It is recommended not to exceed 7 days after storage at 2~8°C, and no more than 30 days for -15°C below.
Notice and Disclaimer
Activity is competitively inhibited by arginine and lysine, and metal ion chelating agents such as EDTA also inhibit enzyme activity.
The product is hygroscopic and should be taken after equilibration at room temperature.
The product is for research use only and should not be used for diagnosis or treatment of animals or humans.
If you want to know more about carboxypeptidase b function, please visit our website.
As one of biopharmaceutical manufacturing companies, we will do our best to meet all the needs of customers.
We can also offer sorts of Recombinant Human Insulin-like Growth Factorfor sale, if you are interested, please leave us a message.
Отправить запрос, связаться с поставщиком
Другие товары поставщика
 |
Pearlwhitein | Gebiotide® Pearlwhitein is a multi peptide complexproduct with dual functions of brightening and anti-aging. Multi-Peptide ComplexMechanism ... |
 |
Anti-acne And Repairing Peptide | Peptides and Acne Sensitive skin is characterized by symptoms of discomfort when exposed to environmental factors. Repair rescue peptides are used... |
 |
Anti-wrinkle Peptide | Anti-wrinkle and anti-aging peptide is is a short chain of amino acids，which are one of the most promising anti-aging treatments because they can p... |
 |
Penicillinase | What Is Penicillinase and What Does It Do? Penicillinase enzyme is widely distributed in various microorganisms, especially in bacteria. As for th... |
 |
Gebiotide® Conopeptide | Gebiotide® Conopeptide is a μ -type conotoxin analog, ultra-fast anti-wrinkle active peptide. Conopeptidecan suppress neuromuscular signal... |
Похожие товары
 |
Company | Продавец: Cubic Sensor and Instrument Co.,Ltd. | Cubic is a leading manufacturer of gas sensors and gas analyzers. With more than 20 years of tech... |
 |
Innovative Methane Emission Monitoring Technology in Oil and Gas Industry in United States | Продавец: Cubic Sensor and Instrument Co.,Ltd. | How is Cubic leveraging innovative TDLAS technology to address environmental concerns of methane ... |
 |
SiSiB® PC9240 | Продавец: Nanjing SiSiB Silicones Co., Ltd | Hexamethyl cyclotrisiloxane SiSiB® PC9240 hexamethylcyclotrisiloxane d3is the simplest membe... |
 |
Lepu Medical AiView V12/V10 Multiparameter Patient Monitor Portable All-in-one Vital Signs Monitor with AI Analysis Diagnosis Touch Screen for Hospital ICU Clinical Ambulance and Home Use | Продавец: SHENZHEN CREATIVE INDUSTRY CO., LTD. | Lepu Medical AiView V12/V10 Multiparameter Patient Monitor Portable All-in-one Vital Signs Monito... |
 |
Lepu LP-M3-11 Cholesterol Analyzer Blood Lipid Meter | Продавец: SHENZHEN CREATIVE INDUSTRY CO., LTD. | Lepu LP-M3-11 Cholesterol Analyzer Blood Lipid Meter In vitro quantitative determination of chol... |
