Recombinant Carboxypeptidase B（with His Tag）
CAS: 9025-24-5
Recombinant carboxypeptidase b（with His Tag） is a lyophilized powder that is isolated and purified from recombinant Pichia pastoris and then freeze-dried, and has the same enzyme activity and specificity as natural carboxypeptidase B.
Recombinant Carboxypeptidase B （with His Tag）Specifications
|
Product Name |
Recombinant Lysyl Endopeptidase (Biochemical Grade) |
|
Appearances |
White or off-white powder |
|
Specific Activity |
≥80U/mg pro |
|
MW |
36kD±3.6kD |
|
Purity |
≥95%（SDS-PAGE） |
|
Protein Content |
20%-60% |
|
Residue DNA |
≤100ng/mg pro |
|
Residue HCP |
≤100ng/mg pro |
|
Optimum pH |
|
|
Solubility |
Soluble in buffer |
|
Storage Conditions |
-15°C below, protected from light，sealed. |
The data should be subject to test report of products.
Carboxypeptidase B Application
Production of various proteins or polypeptides, such as insulin and analogs, cosmetic peptides, antimicrobial peptides, food flavor peptides, and the like.
Quality testing of antibodies.
Determination of the C-terminal amino acid of a protein
Carboxypeptidase Aand BRecommended Method
Protein preparation: The protein was dissolved in a solution of 25 mM Tris-HCl, pH 7.6.
Enzyme preparation: The desired enzyme powder was weighed and dissolved in 25 mM Tris-HCl (pH 7.6) to give an enzyme solution concentration of 1~10 mg/ml.
Reaction recommended enzyme amount: target protein (mass ratio) = 1:50 ~ 1:1000, optimum pH is 7.0~9.0.
Carboxypeptidase B Transport and Storage Stability
Transportation stability: It is transported in an ice pack to keep it active.
Storage stability: The lyophilized powder can be stored for at least 24 months at -15°C below.
25 mM Tris-HCl (pH 7.6) was dissolved and stored at -15°C below, and repeatedly frozen and thawed 10 times without loss of activity.
It is recommended not to exceed 7 days after storage at 2~8°C, and no more than 30 days for -15°C below.
Notice and Disclaimer
Activity is competitively inhibited by arginine and lysine, and metal ion chelating agents such as EDTA also inhibit enzyme activity.
The product is hygroscopic and should be taken after equilibration at room temperature.
The product is for research use only and should not be used for diagnosis or treatment of animals or humans.
If you want to know more about carboxypeptidase b function, please visit our website.
As one of biopharmaceutical manufacturing companies, we will do our best to meet all the needs of customers.
We can also offer sorts of Recombinant Human Insulin-like Growth Factorfor sale, if you are interested, please leave us a message.
在线联系供应商
Other supplier products
 |
Penicillinase | Penicillinase Usesand Penicillinase Function Penicillinase Enzyme is widely distributed in various microorganisms, especially in bacteria. As fo... |
 |
Insulin Degludec | Insulin Degludec, diabetes API, is an ultra-long-acting insulin analogue. Previous studies have shown that it has an ultra-long-term hypoglycemic e... |
 |
Anti-aging Peptide | Best Anti Aging Peptides As people age, their body will produce less collagen and elastin. In the meanwhile, sun damage and stress can also cause ... |
 |
Pearlwhitein | Gebiotide® Pearlwhitein is a multi peptide complexproduct with dual functions of brightening and anti-aging. Multi-Peptide ComplexMechanism ... |
 |
Gebiotide® Antiflamin | Gebiotide® Antiflamin can significantly eliminate skin inflammatory factors, reduce the deepening of skin fine lines by inflammatory factors an... |
Same products
 |
Glycine | 卖方: Shanghai Yifu Food Ingredients Co., Ltd | Glycine is a non-essential amino acid naturally present in the human body and is also a substance... |
 |
Glycine | 卖方: Shanghai Yifu Food Ingredients Co., Ltd | Glycine is an endogenous amino acid in the human body and a fundamental component of proteins. It... |
 |
Glycine | 卖方: Shanghai Yifu Food Ingredients Co., Ltd | Glycine is a food-grade amino acid approved by the state. Due to its high safety, sweet taste, an... |
 |
Glycine | 卖方: Shanghai Yifu Food Ingredients Co., Ltd | Glycine has three reaction centers: amino group, carboxyl group, and methylene group. It has typi... |
 |
Glycine Supply price | 卖方: Shanghai Yifu Food Ingredients Co., Ltd | Physical properties of glycine1. Appearance and OdorAt room temperature, it is a white crystallin... |
