Benzyl penicillin ELISA Test Kit
Benzyl penicillin ELISA Test Kit
Catalog No. LSY-10019
This test kit is based on the competitive enzyme immunoassay for the detection of Benzyl penicillin in samples. The antigens conjugated Benzyl penicillin is pre-coated on the micro-well stripes, Benzyl penicillin in the sample and the conjugated antigens pre-coated on the micro-well stripes compete for the anti-Benzyl penicillin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with Benzyl penicillin concentration in the sample. This value is compared to the standard curve and concentration of Benzyl penicillin in the sample is subsequently obtained.
Sensitivity: 0.1 ppb
Incubator temperature: 25℃
Incubator time: 30min~30min~15min
Detection limit:
Tissue, honey, milk ........................................................................... 2 ppb
Because of some interference, quantification limit of honey, milk sample is 4 ppb
Recovery rate:
Tissue............................................................................................ 75±19%
Honey, milk.................................................................................... 70±17%
Cross-reaction rate:
Benzyl penicillin................................................................................. 100%
Ampicillin........................................................................................... 0.8%
Cloxacillin........................................................................................... 0.2%
Dicloxacillin........................................................................................ 0.1%
Amoxicillin.......................................................................................... 0.1%
Ceftiofu.............................................................................................. 0.1%
1) Micro-well strips: 12 strips with 8 removable wells each
2) 6× standard solution (1 mL each): 0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb and 8.1 ppb
3) Enzyme conjugate (12 mL)............................................................. red cap
4) Antibody working solution (7 mL).................................................. blue cap
5) Substrate A solution (7 mL).......................................................... white cap
6) Substrate B solution (7 mL)......................................................... black cap
7) Stop solution (7 mL).................................................................. yellow cap
8) 20× concentrated washing buffer (40 mL)..................................... white cap
9) 5× concentrated redissolving solution (50 mL)..................... transparent cap
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