Ractopamine ELISA Test Kit

Ractopamine ELISA Test Kit

Catalog No. LSY-10013

The test kit is based on the competitive enzyme immunoassay for the detection of Ractopamine in the sample. The conjugated antigens is pre-coated on the micro-well stripes. The Ractopamine in the sample and the coupling antigens pre-coated on the micro-well stripes compete for anti-Ractopamine antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Ractopamine concentration in the sample. The value is compared to the standard curve and the Ractopamine concentration is subsequently obtained.

Sensitivity: 0.05 ppb

Incubator temperature: 25℃

Incubator time: 30min～15min

Detection limit

Urine.......................................................................................... 0.05 ppb

Tissue……………………………………………………………………… 0.2 ppb

Meat........................................................................................... 0.05 ppb

Liver............................................................................................. 0.1 ppb

Feed............................................................................................ 0.5 ppb

Cross-reactions

Ractopamine................................................................................... 100%

Dobutamine........................................................................................ 1%

Salbutamol..................................................................................... <0.1%

Clenbuterol..................................................................................... <0.1%

Recovery rate

Urine............................................................................................ 95±18%

Tissue.......................................................................................... 75±21%

Feed............................................................................................ 80±25%

1） Micro-well strips: 12 strips with 8 removable wells each

2） 6× standard solution (1 mL each): 0 ppb, 0.05 ppb, 0.15ppb, 0.45 ppb, 1.35 ppb, 4.05 ppb

3） Enzyme conjugate (7 mL)............................................................... red cap

4） Antibody working solution (10 mL)................................................ blue cap

5） Substrate A solution (7 mL)......................................................... white cap

6） Substrate B solution (7 mL)......................................................... black cap

7） Stop solution (7 mL).................................................................. yellow cap

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