Chromatography System

Hanbon advanced chromatography systemshas reliable performance and flexible configuration, and can provide Bio-Lab® laboratory chromatography system, Bio-Pro® pilot&process chromatography system, etc.

ommon Principles Of Process Scale-Up: Linear Scale-Up

Linear scale-up is the most common principle used in process scale-up. By linear scale-up, we mean that we can obtain almost uniform bioprocess chromatographyresults from laboratory scale to pilot results or industrial production level. The core idea is to keep the column height and linear flow rate constant and only scale up the column diameter and volume flow rate. A fixed column height and linear flow rate theoretically ensures the same retention time, the number of CVs used in chromatography and the number of CVs of the eluted sample, maximizing the same purification results. Theoretically, this linear scale-up of the purification separation behavior should be very similar, thus ensuring a successful scale-up of the ion chromatography process. However, some additional factors need to be considered, including a decrease in column tube support (increased pressure drop), column efficiency/column bed instability, sample diffusion (peak spreading), and dead volume of the system and tubing.

Conversion Of Linear Flow Rates (Cm/H) To Volumetric (Ml/Min) Flow Rates

When comparing results from columns of different sizes, it is convenient to express the flow rate as a linear flow rate (cm/h). However, flow rates are often measured as volumetric flow rates (ml/min). In order to convert between linear and volumetric flow rates, one of the following equations can be used .

Linear flow rate (cm/h) to volumetric (ml/min) flow rate

Volumetric flow rate (ml/min) = linear flow rate (cm/h) / 60 x column cross-sectional area (cm2)

=Y/60×π×d2/4

Y = linear flow rate (cm/h)

d = internal diameter of the column (cm)

There is a glass column (inner diameter 1.6 cm), when the linear flow rate is 150 cm/h, what is the volumetric flow rate?

Y=Linear flow rate = 150cm/h

d = inner diameter of the column = 1.6 cm

Volumetric flow rate = 150 x π x 1.6 x 1.6/(60 x 4)

From volumetric flow rate (ml/min) to linear flow rate (cm/h)

Linear flow rate (cm/h) = volumetric flow rate (ml/min) x 60/column cross-sectional area (cm2)

=Z×60×4/π×d2

Z = volumetric flow rate of the column (ml/min)

d = inner diameter of the column (cm)

There is a glass column (inner diameter 0.5 cm), when the volumetric flow rate is 1 ml/min, what is the linear flow rate?

Z = volumetric flow rate of the column = 1 ml/min

d = inner diameter of the column = 0.5 cm

Linear flow rate = 1 x 60 x 4/(π x 0.5 x 0.5) cm/h = 305.6 cm/h

We provide chromatography step by step method, chromatography purification method, chromatography process example, anion exchange chromatography elution, equipment used for chromatography, etc. For more information, please feel free to contact us!

Other supplier products

	Preparative HPLC	Hanbon offers Hanbon preparative HPLCand MPLC from laboratory to industrial level, which are essential tools for the isolation and purification of ...
	Chromatography Column	Hanbon process chromatography columnscan meet the use requirements of most chromatographic fillers. Its good filling effect and high column efficie...
	Biopharm Purification Equipment	Biopharm purification equipment independently developed by Hanbon includes Chromatography columns, Chromatography system, In-line buffer dilution s...
	Preparative Liquid Chromatography System	Hanbon preparative liquid chromatography system has the characteristics of high efficiency, high speed, high automation, and explosion-proof, and i...
	Continuous Chromatographic System	Continuous chromatography systems By implementing this approach, the utilization rate of the ion exchange column can be enhanced, resulting in inc...

供应产品

Same products

	Установки нанесения двухкомпанентного клея Установка для нанесения клея	卖方: 824681	Большой ЖК-экран CAD-файл может быть импортирован и превращен в шаблоны выдачи, а также загружен...
	Inconel alloy Superalloy CNC Machining Services	卖方: 新航金属制品有限公司	英科乃尔合金高温合金 CNC 加工服务简介 英科乃尔合金是一类高性能镍基高温合金，以其出色的强度、抗氧化性和耐高温环境能力而闻名。这些材料广泛应用于航空航天、发电和化学加工行业，这些行业的部件必...
	Titanium Alloy 3D Printing Prototyping Service	卖方: 新航金属制品有限公司	钛合金 3D 打印原型服务简介 钛合金因其出色的强度重量比、出色的耐腐蚀性和在高温环境下的出色性能而受到广泛认可。这些特性使其成为航空航天、医疗、汽车和船舶行业中性能和耐用性至关重要的关键应用的...
	钥匙扣定制	卖方: 中山市狮子五金制品有限公司	定制钥匙扣，原厂，出口多个国家企业钥匙扣 | 皮革钥匙扣 | 促销钥匙扣 | 金属钥匙扣 源头工厂价 12小时免费设计 加急订单可在7-12天内完成 最低订购量为50件 可出口到...
	Eaton Fuller 4300902 Tapered Roller Bearings 53.98×95.2x34mm	卖方: 海双机械有限公司	Bearing Technical DataModel:4300902Structure:Tapered Roller BearingsInner diameter(mm):53.9...