Ractopamine ELISA Test Kit
Ractopamine ELISA Test Kit
Catalog No. LSY-10013
The test kit is based on the competitive enzyme immunoassay for the detection of Ractopamine in the sample. The conjugated antigens is pre-coated on the micro-well stripes. The Ractopamine in the sample and the coupling antigens pre-coated on the micro-well stripes compete for anti-Ractopamine antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Ractopamine concentration in the sample. The value is compared to the standard curve and the Ractopamine concentration is subsequently obtained.
Sensitivity: 0.05 ppb
Incubator temperature: 25℃
Incubator time: 30min～15min
Detection limit
Urine.......................................................................................... 0.05 ppb
Tissue……………………………………………………………………… 0.2 ppb
Meat........................................................................................... 0.05 ppb
Liver............................................................................................. 0.1 ppb
Feed............................................................................................ 0.5 ppb
Cross-reactions
Ractopamine................................................................................... 100%
Dobutamine........................................................................................ 1%
Salbutamol..................................................................................... <0.1%
Clenbuterol..................................................................................... <0.1%
Recovery rate
Urine............................................................................................ 95±18%
Tissue.......................................................................................... 75±21%
Feed............................................................................................ 80±25%
1） Micro-well strips: 12 strips with 8 removable wells each
2） 6× standard solution (1 mL each): 0 ppb, 0.05 ppb, 0.15ppb, 0.45 ppb, 1.35 ppb, 4.05 ppb
3） Enzyme conjugate (7 mL)............................................................... red cap
4） Antibody working solution (10 mL)................................................ blue cap
5） Substrate A solution (7 mL)......................................................... white cap
6） Substrate B solution (7 mL)......................................................... black cap
7） Stop solution (7 mL).................................................................. yellow cap
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