.

BlueGene Biotech

Bovine Carnitine Palmitoyltransferase I ELISA kit

Metabolism

E11C0055 Bovine Carnitine Palmitoyltransferase IELISA kit

Bovine Carnitine Palmitoyltransferase I ELISA kit is suitable for the detection of samples from Bovine species. Carnitine palmitoyltransferase I can also be called arnitine acyltransferase I, CPTI, CAT1, CoA, carnitine acyltransferase i(CCAT), or palmitoyl CoA transferase I, CPT 1, CPT-1.

Specifications of Bovine Carnitine Palmitoyltransferase I ELISA kit

Product Information

E11C0055

Product Name

Bovine Carnitine palmitoyltransferase I ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

Sensitivity

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C

Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1 vial

STANDARD C (0.5mL)

5 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial

Principle of the Assay

The CPT 1 ELISA kit uses an anti-CPT 1 antibody and a CPT 1-HRP conjugate to perform a competitive enzyme immunoassay. The CPT 1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CPT 1 concentration since CPT 1 from samples and CPT 1-HRP conjugate compete for the anti-CPT 1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CPT 1 from the sample, fewer sites are left to bind CPT 1-HRP conjugate. A standard curve is drawn connecting the color's intensity (O.D.) and standard concentration. From this standard curve, the CPT 1 concentration in each sample is extrapolated.

As one of human igg elisa kitsmanufacturers, we are committed to providing the kits with simple and easy operations but accurate and reliable quality to the researchers, and we have won a good reputation from customers all around the world. Many published papers have cited the usage of Shanghai BlueGene BiotechELISA kits.



在线联系供应商

至: Bluegene Biotech
Your E-mail:
消息正文:


Send to other suppliers

Other supplier products

Human Chemokine (C-C motif) Ligand 18 ELISA kit
Human Chemokine (C-C motif) Ligand 18 ELISA kit Immunology NE01C0064 Human Chemokine (C-C motif) Ligand 18 ELISA Kit Human Chemokine (C-C motif) ligand ccl18 elisakit is suitable for the dete...
BlueGene Biotech Canine Endothelin 1 ELISA kit
BlueGene Biotech Canine Endothelin 1 ELISA kit E08E0040 Canine Endothelin 1 ELISAkit Canine Endothelin 1 ELISA kitis suitable for the detection of samples from Canine species. Endothelin 1 ca...
BlueGene Biotech Bovine Cluster Of Differentiation 8 ELISA kit
BlueGene Biotech Bovine Cluster Of Differentiation 8 ELISA kit E11C0007 Bovine Cluster Of Differentiation 8 Bluegene ELISA kit Bovine Cluster of Differentiation 8 insulin ELISAkit is suitable for the detecti...
BlueGene Biotech Canine Brain Derived Neurotrophic Factor ELISA kit
BlueGene Biotech Canine Brain Derived Neurotrophic Factor ELISA kit E08B0029 Canine Brain Derived Neurotrophic Factor ELISA kit Canine Brain Derived Neurotrophic Factor BDNFELISA kitis suitable for the detection ...
BlueGene Biotech Sheep Anti Interleukin 10 ELISA kit
BlueGene Biotech Sheep Anti Interleukin 10 ELISA kit Specifications of Sheep Anti Interleukin 10 ELISAkit Product Information E14A0081 Product Name Sheep Anti Inter...
供应产品

Same products

BlueGene Biotech Sheep Cluster of Differentiation 4 ELISA kit
BlueGene Biotech Sheep Cluster of Differentiation 4 ELISA kit 卖方: Bluegene Biotech E14C0004 Sheep Cluster of Differentiation 4 ELISA kit Sheep Cluster of Differentiation 4 ELISA...
BlueGene Biotech Sheep Anti Interleukin 10 ELISA kit
BlueGene Biotech Sheep Anti Interleukin 10 ELISA kit 卖方: Bluegene Biotech Specifications of Sheep Anti Interleukin 10 ELISAkit Product Information E1...
BlueGene Biotech Chicken Total Adiponectin ELISA kit
BlueGene Biotech Chicken Total Adiponectin ELISA kit 卖方: Bluegene Biotech E12A0125 Chicken Total Adiponectin ELISA kit Chicken Total Adiponectin ELISA kit (a knid of ch...
BlueGene Biotech Bovine Decorin ELISA kit
BlueGene Biotech Bovine Decorin ELISA kit 卖方: Bluegene Biotech E11D0145 Bovine Decorin ELISA kit Bovine Decorin ELISAkit is suitable for the detection of sam...
BlueGene Biotech Bovine C Type Natriuretic Peptide ELISA kit
BlueGene Biotech Bovine C Type Natriuretic Peptide ELISA kit 卖方: Bluegene Biotech E11C0339 Bovine C Type Natriuretic PeptideELISA kit Bovine C Type Natriuretic Peptide ELISAkit...