Lincomycin ELISA Test Kit

Lincomycin ELISA Test Kit

Catalog No. LSY-10045

The test kit is based on the competitive enzyme immunoassay for the detection of Lincomycin in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Lincomycin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Lincomycin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Lincomycin in it. This value is compared to the standard curve and the Lincomycin residues is subsequently obtained.

 

Sensitivity:  0.1 ppb

Incubator temperature:  25℃

Incubator time:  30min15min

Detection limit

Tissue.......................................................................................... 0.4 ppb

Feed............................................................................................... 1 ppb

Honey............................................................................................. 2 ppb

Cross-reaction rate

Lincomycin...................................................................................... 100%

Recovery rate

    Tissue ………………………………………………………………….100%±25%

Feed............................................................................................ 95±25%

Honey..................................................................................... 100%±25%

 

1)   Micro-well strips: 12 strips with 8 removable wells each

2)   6× standard solution (1 mL each): 0 ppb, 0.1ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb,8.1 ppb

3)   Enzyme conjugate (7 mL)............................................................... red cap

4)   Antibody working solution (7 mL).................................................. blue cap

5)   Substrate A solution (7 mL)......................................................... white cap

6)   Substrate B solution (7 mL)......................................................... black cap

7)   Stop solution (7 mL).................................................................. yellow cap

8)   20x concentrated washing buffer (40 mL) ................................... white cap

9)   20× concentrated sample extraction solution (50 mL) ………………transparent cap



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