BlueGene Biotech Canine β Cell Leukemia/Lymphoma 2 ELISA kit
E08B0033 Canine β Cell Leukemia/Lymphoma 2 ELISA kit
Canine β Cell Leukemia/Lymphoma 2 ELISA kit is suitable for the detection of samples from Canine species. β Cell Leukemia/Lymphoma 2 can also be called BCL2L4, Bax Zeta, Apoptosis regulator BAX, Bcl-2-like protein 4, BCL 2, B-cell leukemia/lymphoma 2, Bcl-2 alpha, Apoptosis regulator Bcl-2, Bcl-2-like protein 1, Bcl-2-associated X protein, Bcl-2 homologous antagonist/killer, Bcl-2-related ovarian killer protein, Bcl-w, Bcl-xL, Bcl-xS, Protein phosphatase 1 regulatory subunit 52, PPP1R5.
Specifications of Canine β Cell Leukemia/Lymphoma 2 ELISA kit
|
Product Information |
|
|
E08B0033 |
|
|
Product Name |
Canine βCell Leukemia/Lymphoma 2 ELISA kit |
|
Species |
Canine |
|
Product Size |
48 Tests / 96 Tests |
|
Concentration |
10-25 ng/mL |
|
Sensitivity |
|
|
Principal |
Competitive ELISA |
|
Sample Volume |
100 ul |
|
Sample Type |
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
|
Assay Time |
90 minutes |
|
Platform |
Microplate Reader |
|
Conjugate |
HRP |
|
Detection Method |
Colorimetric |
|
Storage |
2-8°C |
|
Kit Components |
||
|
MATERIALS |
SPECIFICATION |
QUANTITY |
|
MICROTITER PLATE |
96 wells |
stripwell |
|
ENZYME CONJUGATE |
1 vial |
|
|
STANDARD A (0.5mL) |
0 ng/mL |
1 vial |
|
STANDARD B (0.5mL) |
1 vial |
|
|
STANDARD C (0.5mL) |
1 ng/mL |
1 vial |
|
STANDARD D (0.5mL) |
1 vial |
|
|
STANDARD E (0.5mL) |
5 ng/mL |
1 vial |
|
STANDARD F (0.5mL) |
10 ng/mL |
1 vial |
|
SUBSTRATE A |
6 mL |
1 vial |
|
SUBSTRATE B |
6 mL |
1 vial |
|
STOP SOLUTION |
6 mL |
1 vial |
|
WASH SOLUTION (100 x) |
10 mL |
1 vial |
|
BALANCE SOLUTION |
3 mL |
1 vial |
Principle of the Assay
BCL2 ELISAapplies the competitive enzyme immunoassay technique utilizing an anti-BCL 2 antibody and an BCL 2-HRP conjugate. The assay sample and buffer are incubated together with BCL 2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BCL 2 concentration since BCL 2 from samples and BCL 2-HRP conjugate compete for the anti-BCL 2 antibody binding site. Since the number of sites is limited, as more sites are occupied by BCL 2 from the sample, fewer sites are left to bind BCL 2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BCL 2 concentration in each sample is interpolated from this standard curve.
Quality Control on Canine β Cell Leukemia/Lymphoma 2 ELISA kit
|
Coefficient of Variance |
Intra Variation% ＜10% |
|
|
Inter Variation% ＜12% |
||
|
Recovery |
86-107% |
|
|
Linearity |
Diluent Ratio |
Range % |
|
1:02 |
86-109 |
|
|
1:04 |
83-111 |
|
|
1:08 |
83-115 |
|
|
Specificity/ Cross-reactivity |
No significant cross-reactivity or interference between BCL 2 and analogues was observed. |
|
Elisa kit supplier Shanghai BlueGene Biotech is a company with research, production, and sale in one, our product's range includes Enzyme-Linked Immunosorbent Assay (ELISA) kits and registered trademark "cgbio®" ELISA Kits, proteins, antibodies, plasmids, and other reagents, such as His-tag Protein Purification kit. We offer thousands of ELISA kits for researchers worldwide since 2008. As one elisa kit manufacturer, we are committed to providing the kits with simple and easy operations but accurate and reliable quality to the researchers, and we have won a good reputation from customers all around the world. Many published papers have cited the usage of BlueGeneELISAkits.
We can also offer sorts of meth residue test kitsfor sale, if you have needs, please contact us.
在线联系供应商
Other supplier products
 |
ELISA Kits | The elisa kit companyShanghai BlueGene Biotechhas focused on the research and development of ELISA kits, especially for more than a decade. Our ... |
 |
BlueGene Biotech Bovine Cluster Of Differentiation 8 ELISA kit | E11C0007 Bovine Cluster Of Differentiation 8 Bluegene ELISA kit Bovine Cluster of Differentiation 8 insulin ELISAkit is suitable for the detecti... |
 |
BlueGene Biotech Canine Collagen Type II Alpha 1 ELISA kit | E08C0323 Canine Collagen Type II Alpha 1 ELISA kit Canine Collagen Type II Alpha 1 ELISA kit is suitable for the detection of samples from Canin... |
 |
Rat Alpha Amylase ELISA kit | E02A0010 Rat Alpha Amylase ELISA kit Rat Alpha Amylase ELISAkit is suitable for the detection of samples from Rat species. Alpha Amylase can als... |
 |
BlueGene Biotech Canine Brain Derived Neurotrophic Factor ELISA kit | E08B0029 Canine Brain Derived Neurotrophic Factor ELISA kit Canine Brain Derived Neurotrophic Factor BDNFELISA kitis suitable for the detection ... |
Same products
 |
BuyTadalafil (CAS ) powder bulk | 卖方: Aea.ltd | 1.Reviews of Tadalafil Powder Tadalafil powder, commonly marketed under the brand name Cialis, i... |
 |
BlueGene Biotech Sheep Cluster of Differentiation 4 ELISA kit | 卖方: Bluegene Biotech | E14C0004 Sheep Cluster of Differentiation 4 ELISA kit Sheep Cluster of Differentiation 4 ELISA... |
 |
BlueGene Biotech Sheep Anti Interleukin 10 ELISA kit | 卖方: Bluegene Biotech | Specifications of Sheep Anti Interleukin 10 ELISAkit Product Information E1... |
 |
BlueGene Biotech Chicken Total Adiponectin ELISA kit | 卖方: Bluegene Biotech | E12A0125 Chicken Total Adiponectin ELISA kit Chicken Total Adiponectin ELISA kit (a knid of ch... |
 |
BlueGene Biotech Bovine Decorin ELISA kit | 卖方: Bluegene Biotech | E11D0145 Bovine Decorin ELISA kit Bovine Decorin ELISAkit is suitable for the detection of sam... |
