BlueGene Biotech Canine β Cell Leukemia/Lymphoma 2 ELISA kit
E08B0033 Canine β Cell Leukemia/Lymphoma 2 ELISA kit
Canine β Cell Leukemia/Lymphoma 2 ELISA kit is suitable for the detection of samples from Canine species. β Cell Leukemia/Lymphoma 2 can also be called BCL2L4, Bax Zeta, Apoptosis regulator BAX, Bcl-2-like protein 4, BCL 2, B-cell leukemia/lymphoma 2, Bcl-2 alpha, Apoptosis regulator Bcl-2, Bcl-2-like protein 1, Bcl-2-associated X protein, Bcl-2 homologous antagonist/killer, Bcl-2-related ovarian killer protein, Bcl-w, Bcl-xL, Bcl-xS, Protein phosphatase 1 regulatory subunit 52, PPP1R5.
Specifications of Canine β Cell Leukemia/Lymphoma 2 ELISA kit
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Product Information |
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E08B0033 |
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Product Name |
Canine βCell Leukemia/Lymphoma 2 ELISA kit |
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Species |
Canine |
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Product Size |
48 Tests / 96 Tests |
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Concentration |
10-25 ng/mL |
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Sensitivity |
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Principal |
Competitive ELISA |
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Sample Volume |
100 ul |
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Sample Type |
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
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Assay Time |
90 minutes |
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Platform |
Microplate Reader |
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Conjugate |
HRP |
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Detection Method |
Colorimetric |
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Storage |
2-8°C |
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Kit Components |
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MATERIALS |
SPECIFICATION |
QUANTITY |
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MICROTITER PLATE |
96 wells |
stripwell |
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ENZYME CONJUGATE |
1 vial |
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STANDARD A (0.5mL) |
0 ng/mL |
1 vial |
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STANDARD B (0.5mL) |
1 vial |
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STANDARD C (0.5mL) |
1 ng/mL |
1 vial |
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STANDARD D (0.5mL) |
1 vial |
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STANDARD E (0.5mL) |
5 ng/mL |
1 vial |
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STANDARD F (0.5mL) |
10 ng/mL |
1 vial |
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SUBSTRATE A |
6 mL |
1 vial |
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SUBSTRATE B |
6 mL |
1 vial |
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STOP SOLUTION |
6 mL |
1 vial |
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WASH SOLUTION (100 x) |
10 mL |
1 vial |
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BALANCE SOLUTION |
3 mL |
1 vial |
Principle of the Assay
BCL2 ELISAapplies the competitive enzyme immunoassay technique utilizing an anti-BCL 2 antibody and an BCL 2-HRP conjugate. The assay sample and buffer are incubated together with BCL 2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BCL 2 concentration since BCL 2 from samples and BCL 2-HRP conjugate compete for the anti-BCL 2 antibody binding site. Since the number of sites is limited, as more sites are occupied by BCL 2 from the sample, fewer sites are left to bind BCL 2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BCL 2 concentration in each sample is interpolated from this standard curve.
Quality Control on Canine β Cell Leukemia/Lymphoma 2 ELISA kit
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Coefficient of Variance |
Intra Variation% ＜10% |
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Inter Variation% ＜12% |
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Recovery |
86-107% |
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Linearity |
Diluent Ratio |
Range % |
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1:02 |
86-109 |
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1:04 |
83-111 |
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1:08 |
83-115 |
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Specificity/ Cross-reactivity |
No significant cross-reactivity or interference between BCL 2 and analogues was observed. |
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Elisa kit supplier Shanghai BlueGene Biotech is a company with research, production, and sale in one, our product's range includes Enzyme-Linked Immunosorbent Assay (ELISA) kits and registered trademark "cgbio®" ELISA Kits, proteins, antibodies, plasmids, and other reagents, such as His-tag Protein Purification kit. We offer thousands of ELISA kits for researchers worldwide since 2008. As one elisa kit manufacturer, we are committed to providing the kits with simple and easy operations but accurate and reliable quality to the researchers, and we have won a good reputation from customers all around the world. Many published papers have cited the usage of BlueGeneELISAkits.
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