BlueGene Biotech Canine Ferritin ELISA kit
E08F0010 Canine Ferritin ELISA kit
Canine Ferritin ELISAkit is suitable for the detection of samples from Canine species. Ferritin can also be called FE.
Specifications of Canine Ferritin ELISA Testkit
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Product Information |
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E08F0010 |
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Product Name |
Canine Ferritin ELISA kit |
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Species |
Canine |
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Product Size |
48 Tests / 96 Tests |
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Concentration |
25-500 ng/mL |
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Sensitivity |
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Principal |
Competitive ELISA |
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Sample Volume |
100 ul |
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Sample Type |
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
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Assay Time |
90 minutes |
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Platform |
Microplate Reader |
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Conjugate |
HRP |
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Detection Method |
Colorimetric |
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Storage |
2-8°C |
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Kit Components |
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MATERIALS |
SPECIFICATION |
QUANTITY |
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MICROTITER PLATE |
96 wells |
stripwell |
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ENZYME CONJUGATE |
1 vial |
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STANDARD A (0.5mL) |
0 ng/mL |
1 vial |
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STANDARD B (0.5mL) |
25 ng/mL |
1 vial |
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STANDARD C (0.5mL) |
50 ng/mL |
1 vial |
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STANDARD D (0.5mL) |
100 ng/mL |
1 vial |
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STANDARD E (0.5mL) |
250 ng/mL |
1 vial |
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STANDARD F (0.5mL) |
500 ng/mL |
1 vial |
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SUBSTRATE A |
6 mL |
1 vial |
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SUBSTRATE B |
6 mL |
1 vial |
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STOP SOLUTION |
6 mL |
1 vial |
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WASH SOLUTION (100 x) |
10 mL |
1 vial |
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BALANCE SOLUTION |
3 mL |
1 vial |
Principle of the Assay
FE ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-FE antibody and an FE-HRP conjugate. The assay sample and buffer are incubated together with FE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FE concentration since FE from samples and FE-HRP conjugate compete for the anti-FE antibody binding site. Since the number of sites is limited, as more sites are occupied by FE from the sample, fewer sites are left to bind FE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FE concentration in each sample is interpolated from this standard curve.
Quality Control on Canine Ferritin ELISA kit
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Coefficient of Variance |
Intra Variation% ＜10% |
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Inter Variation% ＜12% |
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Recovery |
92-108% |
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Linearity |
Diluent Ratio |
Range % |
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1:02 |
87-113 |
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1:04 |
82-116 |
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1:08 |
84-115 |
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Specificity/Cross-reactivity |
No significant cross-reactivity or interference between FE and analogues was observed. |
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Now the ferritin elisa kit priceis reasonable, if you have needs, please contact us.
BluegeneBiotechhas been committed to R&D of ELISA kits, making our ELISA kits more convenient for researchers to use on the premise of guaranteeing the quality, thus assisting scientific research for our customers. So far, we have received 1200 published papers from researchers with a total of about 500 kinds of ELISA Kits that have been cited. Bluegene ELISA kit has the advantages of high specificity, high sensitivity, and high stability.
If you want to know more kinds of host cell protein elisa kits, please visit our website.
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